Paraffinization
For paraffin sections, trim until you reach your sample (50u) and freeze samples at -200c.
After samples cool, cut sections, before they heat back to room temp, on a fresh part of the knife.
Paraffin for post-hatch Tissues (2+2 Days)
| 1. | PFA 4% | 48h RT |
| 2. | 70% EToH | 1h |
| 3. | 95% EToH | 1h X 3 |
| 4. | Isopropyl Alchohol | 1h X 2 |
| O/N on RT | ||
| 1. | Xylen: Isopropyl Alchohol (50:50) | 1h |
| 2. | Xylen | 1hX 3 |
| 3. | Paraffin | 1~1.5h X 3 at 560 |
| 4. | Sections at 6-8u |
Paraffin for chick embryos
| 1. | PFA 4% | O/N at 40C (or 4h at RT) |
| 2. | PBS | 3X50 |
| 3. | EToH 30% | 1X100 |
| 4. | EToH 70% | 1X100 |
| 5. | EToH 90% | 1X100 |
| 6. | EToH 100% | 2X150 |
| 7. | Xylen | 2X150 |
| 8. | Paraffin | 1X300 |
| 9. | Paraffin | 2X1h |