Postdoctoral position in Structural Biochemistry in protein complexes

A postdoctoral research position is available in the research group of Xavier Manival at the University of Lorraine (IMoPA laboratory, http://www.imopa.cnrs.fr/, Nancy, France) as part of a dynamic team (co-directed by B. Charpentier and I. Motorine) investigating the structure and function of RNA and RNP. snoRNPs are present in Archaea and Eukarya and are among the most ancient non-coding RNPs. They are divided into two families, the box C/D and box H/ACA. snoRNPs play essential roles during ribosomal RNA biogenesis. Most of them catalyze sequence-specific modifications of the rRNA, 2'-O-methylation for the C/D snoRNP and pseudo-uridinylation for the H/ACA one.

Job Description

The postdoctoral project is to decipher the mechanism of the box C/D snoRNP biogenesis by characterizing, at the functional and structural level, assembly pre-complexes to elucidate the role of the assembly machinery on the box C/D snoRNP components. Solution NMR spectroscopy and X-ray diffraction will be complemented with cryo-microscopy building on our previous expertise with these RNA-protein systems (Quinternet et al., 2016; Bizarro et al., 2014; Rothe et al., 2014; Back et al., 2013).

The successful candidate will join an interdisciplinary team of molecular, cellular, and structural biologists. The project will be carried out in close collaboration with other groups as part of an ANR network. We offer a biological/medical environment with structural infrastructure ideal for studying the relationship between structure and function. Our facilities for integrated structural biology include 600 MHz NMR spectrometer with cryogenic probe head, a high-throughput crystallization screening (Mosquito and TECAN robots), X-ray diffractometer (X8-Proteum Bruker), and a biophysics core facility (ITC, DLS, CD, SPR, etc).

The research position is co-funded by french national agency for research (ANR) and the great-East region for 1 year (possibility of 6 more months), starting as soon as possible. Salary will be in the range of €31K/year (gross).

 

Required Technical Skills

We are looking for a colleague that is highly motivated, scientifically independent and creative, and capable of contributing to a team environment. Only candidates holding a PhD obtained less than 2 years ago are eligible for post-doctoral positions. Interested applicants require skills in structural biology, biochemistry, or related fields. Applicants must have an excellent grasp of up-to-date molecular techniques, as well as the production and purification of recombinant proteins from E. coli and/or other organisms (as yeast or insect cells for example) for structural biology. Expertise in at least one of the following areas is desired: protein or RNA biochemistry, X-ray diffraction, and cryo-microscopy.

 

Contact

Please submit your application by email (xavier.manival@univ-lorraine.fr) with a cover letter summarizing current and future research interest, a CV publication list, and contact information for at least two references including one from your present work place.

 

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Dr Xavier Manival 
CR1, CNRS 
Biopôle de l'Université de Lorraine, Campus biologie-santé, Faculté de Médecine
Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA)
UMR 7365 CNRS-Université de Lorraine
Equipe ARN, RNP, maturation-structure-fonction
Groupe de Biologie Moléculaire et Structurale
9 avenue de la Forêt de Haye
BP 20199
54505 Vandoeuvre-Lès-Nancy Cedex