The budding yeast adapts its physiology to radical external and internal fluctuations in a rapid and robust manner. It survives exposure to toxic substances; consumes a variety of nitrogen and carbon sources; lives both as a haploid or diploid organism and carries out both mitosis and meiosis. Uncovering the mechanisms underlying its robustness in the face of extreme environments, genetic mutations and life phases motivates many explorations of this eukaryotic cell. Indeed, vast amounts of data have been collected on transcriptional responses in Saccharomyces cerevisiae. However, only a handful of pioneering studies describe the dynamics of proteins in response to external stimuli, despite the fact that regulation of protein levels and localization is an essential part of such responses. Here we have built a platform to house data that we collect on proteome plasticity.
The data presented here is a result of systematically tracking the localization and abundance of 5330 yeast proteins at single-cell resolution under three different stress conditions (DTT, H2O2 and nitrogen starvation) using the GFP-tagged yeast library. This data was also published in: "A novel single-cell screening platform reveals proteome plasticity during yeast stress responses" (Breker et al, 2013). The database will be updated as more stress conditions become available.
Additional information on the construction and utility of LoQAtE, please enter this link: